Mouse Anti-Histone Recombinant Antibody (clone BWA3)
CAT#: FAMAB-0162YC
Histones are frequent targets of self-reactive antibodies during autoimmune syndromes. Provided is a mouse anti-histone antibody, named BWA3. BWA3 binds an epitope corresponding to a region of high sequence similarity between H2A and H4 (residues 1-20 and 1-29, respectively).
Tested Data
SDS-PAGE
Figure 1 Mouse Anti-Histone Recombinant Antibody (clone BWA3) (FAMAB-0162YC) in SDS-PAGE
SDS-PAGE analysis of FAMAB-0162YC in non-reduced (Lane 1) and reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result, different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.
The purity of FAMAB-0162YC was greater than 95% as determined by SDS-PAGE.
SEC-HPLC
Figure 2 Mouse Anti-Histone Recombinant Antibody (clone BWA3) (FAMAB-0162YC) in HPLC
The purity of FAMAB-0162YC was greater than 90% as determined by SEC-HPLC.
WB
Figure 3 Mouse Anti-Histone Recombinant Antibody (clone BWA3) (FAMAB-0162YC) in WB
Western blot analysis of FAMAB-0162YC was performed by loading recombinant human histone H4 protein.
TAB-388CT incubation concentration: 2 ng/μL.
The secondary antibody: HRP-goat anti-mouse IgG
Lane 1: Reducing Antigen (0.1 μg)
Lane 2: Reducing Antigen (0.3 μg)
Lane 3: Reducing Antigen (0.6 μg)
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Specifications
- Host Species
- Mouse
- Type
- Mouse IgG1, κ
- Specificity
- Mouse Histone
- Species Reactivity
- Mouse
- Clone
- BWA3
- Applications
- IF
- Related Disease
- Autoimmune syndromes
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- H; Histone; Alkaline protein
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To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# FAMAB-0162YC, RRID: AB_3111316)
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "Clone BWA3"
- CAT
- Product Name
See other products for "Histone"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-465 | Recombinant Anti-Histone Antibody | WB, IF, FuncS | IgG |
| FAMAB-0160YC | Mouse Anti-Histone Recombinant Antibody (clone LG2-1) | IF | Mouse IgG2a, κ |
| FAMAB-0161YC | Mouse Anti-Histone Recombinant Antibody (clone LG2-2) | IF | Mouse IgG2a, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHH-465-F(E) | Recombinant Human Anti-Histone Antibody Fab Fragment | ELISA, WB, FC, FuncS | Fab |
| FAMAB-0160-YC-F(E) | Mouse Anti-Histone Recombinant Antibody (clone LG2-1); Fab Fragment | IF | Mouse Fab |
| FAMAB-0161-YC-F(E) | Mouse Anti-Histone Recombinant Antibody (clone LG2-2); Fab Fragment | IF | Mouse Fab |
| FAMAB-0162-YC-F(E) | Mouse Anti-Histone Recombinant Antibody (clone BWA3); Fab Fragment | IF | Mouse Fab |
| FAMAB-0175CQ-F(E) | Mouse Anti-Histone Recombinant Antibody (clone MRL-Histone7); Fab Fragment | ELISA | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHH-465-S(P) | Recombinant Human Anti-Histone Antibody scFv Fragment | ELISA, WB, IP, FuncS | scFv |
| FAMAB-0160-YC-S(P) | Mouse Anti-Histone Recombinant Antibody (clone LG2-1); scFv Fragment | IF | Mouse scFv |
| FAMAB-0161-YC-S(P) | Mouse Anti-Histone Recombinant Antibody (clone LG2-2); scFv Fragment | IF | Mouse scFv |
| FAMAB-0162-YC-S(P) | Mouse Anti-Histone Recombinant Antibody (clone BWA3); scFv Fragment | IF | Mouse scFv |
| FAMAB-0175CQ-S(P) | Mouse Anti-Histone Recombinant Antibody (clone MRL-Histone7); scFv Fragment | ELISA | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0175CQ | Mouse Anti-Histone Recombinant Antibody (clone MRL-Histone7) | ELISA | Mouse IgM, κ |
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